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Field trials results
We continue to test Tryptonite with universities, testing centers and customers to demonstrate its effectiveness and understand its potential.
Table grape
Cherries
Soybean
What it can do
for your crops
From Agridaeus research, an innovative formulation of a nutrient that amplifies the biosynthesis of auxins (IAA), serotonin and melatonin in plants starting from the natural precursor L-Tryptophan.
The secret of its effectiveness lies in the combination of its components:
▬ Energy ▬ Antioxidants ▬ Enzyme cofactors ▬ Osmolytes
Tryptonite doesn't provide a specific molecule, but it provides plants with all the elements necessary for the biosynthesis of molecules essential for proper development. The plant itself directs metabolic processes according to its own needs, allowing it to maintain maximum efficiency and productivity in all environmental conditions.
FRUIT SET
Auxins regulate pollen germination and promote rapid, straighter, and thinner pollen tube growth, facilitating elongation.
Auxins also increase the flow of calcium and boron, which are essential for proper fruit set.
TRYPTONITE stimulates the biosynthesis of melatonin and serotonin, powerful antioxidants that protect pollen tubes, ovaries, and embryos, thus enabling regular fruit development.
FLOWER & FRUIT
DROP
Numerous studies highlight increased abscisic acid (ABA) content, reduced indole-3-acetic acid (IAA), and reduced sugar and nutrient flow as causes of flower and fruit drop.
Abiotic stresses (temperature, salinity, drought, microwounds) and the genetic characteristics of some varieties induce the biosynthesis of abscisic acid at the expense of other hormones.
TRYPTONITE stimulates the synthesis of endogenous auxins, which inhibit the negative effects of abscisic acid.
It also increases photosynthetic efficiency and water use, and possesses a high antioxidant capacity, both directly and by stimulating antioxidant enzymes that protect the plant from the negative effects of environmental and biotic stress.
FRUIT ENLARGMENT
TRYPTONITE stimulates post-fruit set cell multiplication; a greater number of cells predisposes fruit enlargement. It also promotes cell distension and membrane elasticity.
It regulates cell turgor and water absorption; it improves the transport of nutrients (N, P, K, Mg, and, above all, Boron and Calcium), which are essential for the stability and resistance of cell membranes.
It maintains the vascular function (xylem, phloem) of the peduncle.
MILLERANDAGE
Millerandage is a grapevine disorder in which clusters contain a mix of normal berries and small seedless berries, leading to irregular ripening and a reduction in quality and yield.
The causes include: heat stress during the pre-flowering and flowering periods disrupts pollen development, ovule fertilization, and fruit set, increasing millenellation rates; nutritional deficiencies, particularly boron and zinc, as well as poor water status before flowering, compromise reproductive processes and increase the disorder. Some varieties are more susceptible to the problem.
This phenomenon is increasingly significant with climate change.
TRYPTONITE protects the vine from all possible causes, drastically reducing the problem to almost zero.
PHYSIOPATHIES
Calcium is the key macronutrient in cell wall structure, membrane stability, signaling, and stress responses.
Tip burn and blossom-end rot are among the most important plant diseases, manifesting as necrosis of leaf margins or fruit tips due to a localized calcium deficiency, even when the plant's total calcium content is adequate.
TRYPTONITE improves calcium absorption and translocation to both leaves and fruits, regulates water balance, and maintains the efficiency of xylem and phloem vessels, all to the benefit of calcium nutrition.
nutrient accumulation
Auxins increase root uptake, transport, and accumulation of the nutrients K+, Ca2+, Mg2+, and B in the above-ground parts of plants.
Auxins also increase protein biosynthesis and the efficiency of nutrient and sugar transport to storage organs (seeds, fruits, roots).
ROOTING
Auxins stimulate the growth of primary root tips, induce the emergence of lateral roots, and stimulate the activity of microflora, increasing the plant's water and nutrient uptake capacity.
Furthermore, auxins promote symbiosis with nitrogen-fixing plants and the growth of root tubers, promoting nutrient accumulation in the roots and increasing their efficiency.
cracking
TRYPTONITE performs numerous activities within the fruit that prevent cracking: it improves calcium transport, increasing the mechanical resistance of cell walls; it promotes cell multiplication, distension and elasticity of cell membranes, with clear benefits for the structural stability of the fruit; it regulates cellular osmotic balance; and it increases the enzymes that protect membranes.
WATER stress
TRYPTONITE activates physiological processes in the plant that increase resistance to water stress: it regulates cellular osmotic balance; modulates stomatal opening; activates the antioxidant enzymes SOD, CAT, POD, APX; activates membrane-protecting enzymes, increases water absorption capacity.
TERMAL stress
The application of TRYPTONITE stimulates the biosynthesis of key metabolites in plants, such as auxin (IAA), melatonin, and indolamines, which together improve plant adaptation to heat stress. Exogenous application of tryptophan stabilizes altered metabolism, improves antioxidant capacity, and coordinates hormonal interaction, leading to improved stress resistance in crops. Overexpression of tryptophan decarboxylase or related genes increases levels of protective compounds, increasing tolerance without compromising yields.
Increasing IAA and related metabolites supports thermotolerance, recovery, and antioxidant defenses.
SALINITY stress
Auxin (indole-3-acetic acid, IAA) and melatonin are both responsible for regulating growth and stress responses. Applying TRYPTONITE increases auxin levels, improves osmotic regulation, enhances the activity of antioxidant enzymes (such as peroxidase and superoxide dismutase), and reduces oxidative damage by neutralizing reactive oxygen species. It also modulates ion transport, helping maintain a favorable K+/Na+ ratio, and supports cellular structural integrity under salt stress.
Tryptophan synthase and decarboxylase genes are upregulated under salt stress, linking tryptophan metabolism to increased melatonin and auxin production, which in turn improves stress tolerance. Proteomic studies reveal a greater abundance of tryptophan-related enzymes and stress-responsive proteins in plants subjected to salt stress.
HEAVY METALS
STRESS
Melatonin, auxin, and serotonin play multiple roles in protecting plants from heavy metal stress. Melatonin, in particular, enhances antioxidant activity, regulates metal transporter genes, and reduces heavy metal accumulation and oxidative damage in plants. Auxin also supports root development and nutrient uptake, indirectly contributing to stress mitigation.
SPRING FROSTS
The biosynthesis of metabolites such as melatonin and auxin, induced by the application of TRYPTONITE, contributes to adaptation to cold stress by modulating antioxidant defenses, hormonal signaling, and metabolic pathways.
Melatonin enhances antioxidant activity and protects photosynthetic mechanisms under cold stress, leading to a reduction in reactive oxygen species (ROS) and improved crop growth.
Tryptophan synthase and related genes coordinate the interaction between tryptophan, auxin, and ABA, optimizing cold adaptation. Cold-tolerant genotypes show positive regulation of tryptophan metabolism, which supports membrane stability, osmoregulation, and the production of secondary metabolites, improving resilience.
photosynthetic efficiency
TRYPTONITE enhances photosynthetic processes primarily by increasing the synthesis of photosynthetic pigments such as chlorophylls and carotenoids, which are essential for light absorption and energy conversion, thus improving photosynthetic efficiency and providing photoprotection through the xanthophyll cycle. Tryptophan also acts as an auxin precursor, stimulating gene expression (e.g., PSY) involved in pigment biosynthesis.
Metabolites such as auxin, melatonin, and serotonin play a crucial role in plant adaptation to abiotic stress; they regulate stomatal opening and mitigate oxidative damage, thus indirectly supporting photosynthetic performance under adverse conditions.
Furthermore, tryptophan's action extends to the maintenance and repair of photosynthetic complexes, protecting photosystem II, ensuring sustained photosynthetic activity even under conditions of intense light stress.
oil and essential oil yield
The application of TRYPTONITE activates the biosynthesis of auxins (particularly indole-3-acetic acid), melatonin, indole alkaloids, and volatile compounds such as methyl anthranilate, which contribute to plant defense, growth, and the synthesis of aromatic compounds. In oilseed crops such as Brassica napus, tryptophan aminotransferase (TAR)-related genes are linked to both auxin biosynthesis and quantitative trait loci (QTL) for oil content and seed weight, suggesting a genetic connection between tryptophan metabolism and oil biosynthesis.
The application of TRYPTONITE increases essential oil yield and can synergistically enhance the accumulation of carotenoids, powerful antioxidants that reduce lipid peroxidation.
leaf development
TRYPTONITE bioactivates the synthesis of auxin, the hormone essential for leaf initiation, expansion, and morphogenesis, directly impacting leaf growth and development.
Exogenous application of tryptophan increases leaf surface area, chlorophyll content, and photosynthetic capacity, especially under stressful conditions. In Arabidopsis, tryptophan deficiency impairs cell expansion and leaf growth, which can be restored with exogenous tryptophan application but not by auxin alone, highlighting a unique role in cell expansion.
Research
Tryptonite is the fruit of five years of research at Agridaeus.
Collaborations with universities and testing centers in Italy and around the world have allowed us to acquire extensive product expertise, discover its mechanisms of action, application timing, and its potential to stimulate plant metabolic processes.
The results confirm this: Tryptonite can improve the quantity and quality of agricultural production, protect plants from environmental stress, and increase crop profitability.
Auxin
Indole-3-acetic acid (IAA), the main natural auxin in higher plants, has profound effects on plant growth and development. Auxins are involved in numerous processes:
▬ development of flower components;
▬ fruit set and parthenocarpy;
▬ prevention of flower and fruit drop;
▬ cell multiplication;
▬ cell distension;
▬ prevention of cracking;
▬ phloem and xylem differentiation;
▬ vascular flow of nutrients and assimilates;
▬ development of lateral roots;
▬ delay of leaf senescence;
▬ increased resistance to water stress;
▬ increased resistance to heat stress;
▬ increased resistance to salinity.
▬ increased resistance to heavy metals.
Serotonin
Serotonin plays important roles in plant growth and development, including chronoregulation and modulation of reproductive development, control of root and shoot organogenesis, maintenance of plant tissue, delay of senescence, and responses to biotic and abiotic stresses.
Melatonin
In plants, melatonin acts to reduce oxidative stress, promotes germination and seed growth, improves resistance to environmental stress, stimulates the immune system by increasing resistance to pathogens, and modulates circadian rhythms by influencing processes such as the opening and closing of stomata, leaf movement, and flowering.
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